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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | serpin family B member 1 |
| Gene Symbol: | SERPINB1 |
| Synonyms: | EI; LEI; PI2; MNEI; PI-2; HEL57; M/NEI; ELANH2; HEL-S-27 |
| Gene ID: | 1992 |
| UniProt ID: | P30740 |
| Clone ID: | 24GB6545 |
| Immunogen: | Recombinant protein of human SERPINB1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein SERPINB1 encoded by this gene is a member of the serpin family of proteinase inhibitors. Members of this family maintain homeostasis by neutralizing overexpressed proteinase activity through their function as suicide substrates. This protein inhibits the neutrophil-derived proteinases neutrophil elastase, cathepsin G, and proteinase-3 and thus protects tissues from damage at inflammatory sites. Alternative splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-serpin family B member 1 antibody (Cat#63565). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-serpin family B member 1 antibody (Cat#63565, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-serpin family B member 1 antibody (Cat#63565). Serpin family B member 1 expression in wild type (WT) and serpin family B member 1 (SERPINB1) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-serpin family B member 1 antibody (Cat#63565, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of Serpin family B member 1 expression in HepG2 cells using anti-Serpin family B member 1 antibody (Cat#63565, 1:2,000). Green, isotype control; red, Serpin family B member 1. |
 | Immunocytochemical staining of HepG2 cells with anti-Serpin family B member 1 antibody (Cat#63565, 1:1,000). Nuclei were stained blue with DAPI; Serpin family B member 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Medium. Scale bar, 20 μm. |
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