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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | vascular endothelial growth factor D |
| Gene Symbol: | VEGFD |
| Synonyms: | FIGF; VEGF-D |
| Gene ID: | 2277 |
| UniProt ID: | O43915 |
| Clone ID: | 24GB1750 |
| Immunogen: | A synthesized peptide derived from human beta VEGFD |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein VEGFD encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor C. Read-through transcription has been observed between this locus and the upstream PIR (GeneID 8544) locus.
Images
 | Western blotting analysis using anti-Vascular endothelial growth factor D antibody (Cat#62636). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Vascular endothelial growth factor D antibody (Cat#62636, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-Vascular endothelial growth factor D antibody (Cat#62636). Vascular endothelial growth factor D expression in wild type (WT) and Vascular endothelial growth factor D shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Vascular endothelial growth factor D antibody (Cat#62636, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Vascular endothelial growth factor D expression in HepG2 cells using anti-Vascular endothelial growth factor D antibody (Cat#62636, 1:2,000). Green, isotype control; red, Vascular endothelial growth factor D. |
 | Immunocytochemical staining of HepG2 cells with anti-Vascular endothelial growth factor D antibody (Cat#62636, 1:1,000). Nuclei were stained blue with DAPI; Vascular endothelial growth factor D was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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