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| Reactivity: | Human |
| Applications: | WB |
| Host Species: | Mouse |
| Isotype: | IgG1,k |
| Clonality: | Monoclonal antibody |
| Gene Name: | X-linked inhibitor of apoptosis |
| Gene Symbol: | XIAP |
| Synonyms: | API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3 |
| Gene ID: | 331 |
| UniProt ID: | P98170 |
| Clone ID: | 24GB8570 |
| Immunogen: | Recombinant human XIAP |
| Dilution: | WB 1:1,000-1:5,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene XIAP encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11.
Images
 | Western blotting analysis using anti-X-linked inhibitor of apoptosis antibody (Cat#63773). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-X-linked inhibitor of apoptosis antibody (Cat#63773, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-X-linked inhibitor of apoptosis antibody (Cat#63773). X-linked inhibitor of apoptosis expression in wild-type (WT) and X-linked inhibitor of apoptosis (XIAP) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-X-linked inhibitor of apoptosis antibody (Cat#63773, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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