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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | kelch domain containing 3 |
| Gene Symbol: | KLHDC3 |
| Synonyms: | PEAS; dJ20C7.3; KLHDC3 |
| Gene ID: | 116138 |
| UniProt ID: | Q9BQ90 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-382 of human KLHDC3 (NP_476502.1). |
| Dilution: | WB 1:1000-1:5000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.19 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-KLHDC3 antibody |
Background
The protein encoded by this gene contains six repeated kelch motifs that are structurally similar to recombination activating gene 2, a protein involved in the activation of the V(D)J recombination. In mouse, this gene is found to be expressed specifically in testis. Its expression in pachytene spermatocytes is localized to cytoplasma and meiotic chromatin, suggesting that this gene may be involved in meiotic recombination.
Images
 | Western blot analysis of various lysates using KLHDC3 Rabbit pAb (A13741) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of HeLa cells using KLHDC3 Rabbit pAb (A13741) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of PC-12 cells using KLHDC3 Rabbit pAb (A13741) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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