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Anti-KLHL13 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & M |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Kelch-like protein 13 |
Synonyms: | BKLHD2, kelch like 13 (Drosophila), Kelch like protein 13, KIAA1309 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse KLHL13 |
Uniprot ID: | Q80TF4 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 74 kDa / 71 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 800-1: 1200 | placenta, testis |
ICC/IF | Mouse | 1: 100-1: 300 | NIH-3T3 |
Background
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis. The BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex mediates the ubiquitination of AURKB and controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis.
Images
| WB analysis of KLHL13(GB114919). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 800, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| ICC analysis of KLHL13(GB114919)red. Sample: NIH-3T3, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 200, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |