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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | actinin alpha 4 |
| Gene Symbol: | ACTN4 |
| Synonyms: | FSGS; FSGS1; ACTININ-4 |
| Gene ID: | 81 |
| UniProt ID: | O43707 |
| Clone ID: | 25GB535 |
| Immunogen: | A synthesized peptide derived from human alpha Actinin 4 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.
Images
 | Western blotting analysis using anti-alpha Actinin 4 antibody (Cat#71140). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-alpha Actinin 4 antibody (Cat#71140, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-alpha actinin 4 antibody (Cat#71140). Alpha actinin 4 expression in wild type (WT) and alpha actinin 4 (ACTN4) knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-alpha actinin 4 antibody (Cat#71140, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of alpha Actinin 4 expression in HepG2 cells using anti-alpha Actinin 4 antibody (Cat# 71140, 1:2,000). Green, isotype control; red, alpha Actinin 4. |
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