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| Reactivity: | Human |
| Applications: | WB |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | aldehyde dehydrogenase 1 family member A3 |
| Gene Symbol: | ALDH1A3 |
| Synonyms: | ALDH6; MCOP8; RALDH3; ALDH1A6 |
| Gene ID: | 220 |
| UniProt ID: | P47895 |
| Clone ID: | 25GB3800 |
| Immunogen: | Recombinant protein of human ALDH1A3 |
| Dilution: | WB 1:100-1:500 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The gene ALDH1A3 encodes an aldehyde dehydrogenase enzyme that uses retinal as a substrate. Mutations in this gene have been associated with microphthalmia, isolated 8, and expression changes have also been detected in tumor cells. Alternative splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-ALDH1A3 antibody (Cat#71253). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ALDH1A3 antibody (Cat#71253, 1:500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-ALDH1A3 antibody (Cat#71253). ALDH1A3 expression in wild-type (WT) and ALDH1A3 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ALDH1A3 antibody (Cat#71253, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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