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| Reactivity: | Human |
| Applications: | WB |
| Host Species: | Mouse |
| Isotype: | IgG2b |
| Clonality: | Monoclonal antibody |
| Gene Name: | hydroxyacyl-CoA dehydrogenase |
| Gene Symbol: | HADH |
| Synonyms: | HAD; HCDH; HHF4; HADH1; SCHAD; HADHSC; MSCHAD |
| Gene ID: | 3033 |
| UniProt ID: | Q16836 |
| Clone ID: | 25GB675 |
| Immunogen: | Recombinant protein of human HADH |
| Dilution: | WB 1:400-1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15.
Images
 | Western blotting analysis using anti-HADH antibody (Cat#71173). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HADH antibody (Cat#71173, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-HADH antibody (Cat#71173). HADH expression in wild type (WT) and HADH knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HADH antibody (Cat#71173, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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