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Cytokeratin 18 (KRT18) Rabbit pAb (20 μl)
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | keratin 18 |
| Gene Symbol: | KRT18 |
| Synonyms: | K18; CK-18; CYK18; Cytokeratin 18 (KRT18) |
| Gene ID: | 3875 |
| UniProt ID: | P05783 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 127-430 of human Cytokeratin 18 (KRT18) (NP_000215.1). |
| Dilution: | WB 1:1000-1:5000; IHC 1:50-1:100; IF/IC 1:100-1:500 |
| Purification Method: | Affinity purification |
| Concentration: | 2.92 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-KRT18 antibody |
Background
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
Images
 | Western blot analysis of various lysates, using Cytokeratin 18 (KRT18) Rabbit pAb (A1022) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 0.5s. |
 | Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Cytokeratin 18 (KRT18) Rabbit pAb (A1022) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human small intestine using Cytokeratin 18 (KRT18) Rabbit pAb (A1022) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunofluorescence analysis of HepG2 cells using Cytokeratin 18 (KRT18) Rabbit pAb (A1022) at dilution of 1:300 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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