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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | leucine rich repeat containing G protein-coupled receptor 4 |
| Gene Symbol: | LGR4 |
| Synonyms: | DPSL; GPR48; BNMD17; LGR4 |
| Gene ID: | 55366 |
| UniProt ID: | Q9BXB1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 320-540 of human LGR4 (NP_060960.2). |
| Dilution: | WB 1:1000-1:5000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.72 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-LGR4 antibody |
Background
The protein encoded by this gene is a G-protein coupled receptor that binds R-spondins and activates the Wnt signaling pathway. This Wnt signaling pathway activation is necessary for proper development of many organs of the body.
Images
 | Western blot analysis of various lysates using LGR4 Rabbit pAb (A12657) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of paraffin-embedded human placenta using LGR4 Rabbit pAb (A12657) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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