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| Reactivity: | Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | leucine rich repeat containing G protein-coupled receptor 5 |
| Gene Symbol: | LGR5 |
| Synonyms: | FEX; HG38; GPR49; GPR67; GRP49; LGR5/GPR49 |
| Gene ID: | 8549 |
| UniProt ID: | O75473 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 824-907 of human LGR5/GPR49 (NP_003658.1). |
| Dilution: | WB 1:1000-1:5000; IHC 1:100-1:500 |
| Purification Method: | Affinity purification |
| Concentration: | 2.92 mg/mL |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-LGR5 antibody |
Background
The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blot analysis of lysates from Mouse brain using LGR5/GPR49 Rabbit pAb (A10545) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry analysis of paraffin-embedded Rat brain using LGR5/GPR49 Rabbit pAb (A10545) at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat lung using LGR5/GPR49 Rabbit pAb (A10545) at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat ovary using LGR5/GPR49 Rabbit pAb (A10545) at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
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