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| Reactivity: | Human, Rat |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | LIM zinc finger domain containing 2 |
| Gene Symbol: | LIMS2 |
| Synonyms: | LGMD2W; PINCH2; MDRCMTT; PINCH-2; LIMS2 |
| Gene ID: | 55679 |
| UniProt ID: | Q7Z4I7 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-341 of human LIMS2 (NP_001154875.1). |
| Dilution: | WB 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 2.19 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-LIMS2 antibody |
Background
This gene encodes a member of a small family of focal adhesion proteins which interacts with ILK (integrin-linked kinase), a protein which effects protein-protein interactions with the extraceullar matrix. The encoded protein has five LIM domains, each domain forming two zinc fingers, which permit interactions which regulate cell shape and migration. A pseudogene of this gene is located on chromosome 4. Multiple transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blot analysis of various lysates using LIMS2 Rabbit pAb (A13225) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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