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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | lamin B1 |
| Gene Symbol: | LMNB1 |
| Synonyms: | LMN; ADLD; LMN2; LMNB; MCPH26 |
| Gene ID: | 4001 |
| UniProt ID: | P20700 |
| Clone ID: | 5H8V8 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 450-550 of human Lamin B1 (P20700). |
| Dilution: | WB 1:1000-1:6000; IHC 1:200-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.3 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-LMNB1 antibody |
Background
This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants.
Images
 | Western blot analysis of various lysates using Lamin B1 Rabbit mAb (A11495) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry analysis of paraffin-embedded Human brain using Lamin B1 Rabbit mAb (A11495) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Lamin B1 Rabbit mAb (A11495) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon using Lamin B1 Rabbit mAb (A11495) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse colon using Lamin B1 Rabbit mAb (A11495) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
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