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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | mitotic arrest deficient 2 like 1 |
| Gene Symbol: | MAD2L1 |
| Synonyms: | MAD2; HSMAD2; MAD2/MAD2L1 |
| Gene ID: | 4085 |
| UniProt ID: | Q13257 |
| Clone ID: | 5W5U1 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 70-150 of human MAD2/MAD2L1 (Q13257). |
| Dilution: | WB 1:1000-1:2000; IHC 1:100-1:800 |
| Purification Method: | Affinity purification |
| Concentration: | 0.216 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-MAD2L1 antibody |
Background
MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14.
Images
 | Western blot analysis of various lysates using MAD2/MAD2/MAD2L1 Rabbit mAb (A11469) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse kidney using MAD2/MAD2/MAD2L1 Rabbit mAb (A11469) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat spleen using MAD2/MAD2/MAD2L1 Rabbit mAb (A11469) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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