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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, ELISA |
| Host Species: | Rabbit |
| Clonality: | Monoclonal antibody |
| Gene Name: | microtubule associated protein 1 light chain 3 alpha |
| Gene Symbol: | MAP1LC3A |
| Synonyms: | LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3; MAP1LC3A |
| Gene ID: | 84557 |
| UniProt ID: | Q9H492 |
| Clone ID: | 7F5R1 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP1LC3A (Q9H492). |
| Dilution: | WB 1:1000-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.4 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-MAP1LC3A antibody |
Background
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis.
Images
 | Western blot analysis of lysates from NIH/3T3,C6 cells using MAP1LC3A Rabbit mAb (A12319) at 1:1000 dilution. NIH/3T3 cells and C6 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Western blot analysis of lysates from SH-SY5Y cells using MAP1LC3A Rabbit mAb (A12319) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunoprecipitation analysis of 300 μg extracts of NIH/3T3 cells using 3 μg MAP1LC3A antibody (A12319). Western blot was performed from the immunoprecipitate using MAP1LC3A antibody (A12319) at a dilution of 1:1000.NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours. |
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