Your shopping cart is empty!
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | MyoD family inhibitor |
| Gene Symbol: | MDFI |
| Synonyms: | I-MF; I-mfa; MDFI |
| Gene ID: | 4188 |
| UniProt ID: | Q99750 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human MDFI (NP_005577.1). |
| Dilution: | WB 1:500-1:2000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.12 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-MDFI antibody |
Background
This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures.
Images
 | Western blot analysis of various lysates using MDFI Rabbit pAb (A13709) at 1:3000 dilution. |
 | Immunofluorescence analysis of U-2 OS cells using MDFI Rabbit pAb (A13709) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
You may also be interested in:
Related Products
-10%
-16%
