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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | mesoderm posterior bHLH transcription factor 1 |
| Gene Symbol: | MESP1 |
| Synonyms: | bHLHc5; MESP1 |
| Gene ID: | 55897 |
| UniProt ID: | Q9BRJ9 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 149-268 of human MESP1 (NP_061140.1). |
| Dilution: | WB 1:200-1:1000; IHC 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.46 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-MESP1 antibody |
Background
Enables DNA-binding transcription factor activity and transcription cis-regulatory region binding activity. Involved in several processes, including endothelial cell differentiation; heart development; and positive regulation of transcription by RNA polymerase II. Predicted to be part of chromatin. Predicted to be active in nucleus.
Images
 | Western blot analysis of various lysates using MESP1 Rabbit pAb (A10148) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using MESP1 Rabbit pAb (A10148) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using MESP1 Rabbit pAb (A10148) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using MESP1 Rabbit pAb (A10148) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using MESP1 Rabbit pAb (A10148) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining. |
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