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Anti-MOCS3 Rabbit pAb (100 μl)
| Reactivity: | H |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Adenylyltransferase and sulfurtransferase MOCS3 |
Synonyms: | MOCS3, UBA4, Sulfurtransferase MOCS3, Adenylyltransferase MOCS3 |
Immunogen: | Recombinant protein corresponding to Mouse MOCS3 |
Uniprot ID: | A2BDX3 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 49 kDa / 65 kDa |
Product Usage Information
WB | Human | 1: 2000-1: 4000 | heart, liver, brain |
Background
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed.
Images
| Western blot analysis of MOCS3 (GB114240) at dilution of 1: 2000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |