| Reactivity: | M,R & M | 
| Applications: | WB & ICC/IF | 
| Host Species: | Rabbit | 
| Clonality: | Polyclonal | 
| Gene Name: | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5  | 
Synonyms:  | B13, CI 13KD B, Complex I 13kD B, Complex I subunit B13, NDUFA5, NUFM, UQOR13  | 
Immunogen:  | Recombinant protein corresponding to Mouse NDUFA5  | 
Uniprot ID:  | Q9CPP6  | 
Isotype:  | IgG  | 
Purity:  | Affinity purification  | 
Predicted MW. / Observed MW.  | 13 kDa / 13 kDa  | 
Product Usage Information
WB  | Mouse, Rat  | 1:3000-1:5000  | heart, brain, liver  | 
ICC/IF  | Mouse  | 1: 50-1: 200  | NIH-3T3  | 
Background
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Images
  | Western blot analysis of NDUFA5 (GB114241) at dilution of 1: 3000  | 
  | Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH-3T3 cells using NDUFA5(GB114241) at dilution of 1: 50-1: 200  | 
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. | 
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |