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| Reactivity: | H,M & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | NEK7 rabbit polyclonal antibody |
Gene Name: | Serine/threonine-protein kinase Nek7 |
Synonyms: | Never in mitosis A-related kinase 7, NimA-related protein kinase 7, Nek7, NIMA-related kinase 7 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse NEK7 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Cytoskeleton, Nucleus |
Predicted MW. | 35 kDa |
Observed MW. | 35 kDa |
Uniprot ID: | Q8TDX7, Q9ES74, D3ZBE5 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse | 1: 800-1: 1600 | brain |
IHC/IF | Mouse, Rat | 1: 200-1: 300 | brain, Skeletal muscle |
Background
Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1.
Images
| Western blot analysis of Nek7 (GB112643) at dilution of 1: 800 |
| Immunohistochemistry of paraffin embedded mouse brain using Nek7 (GB112643) at dilution of 1: 300 (400x lens) |
| Immunohistochemistry of paraffin embedded mouse skeletal muscle using Nek7 (GB112643) at dilution of 1: 200 (400x lens) |
 | Immunohistochemistry of paraffin embedded rat brain using Nek7 (GB112643) at dilution of 1: 300 (400x lens) |
 | Immunohistochemistry of paraffin embedded rat skeletal muscle using Nek7 (GB112643) at dilution of 1: 200 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |