NFIL3 Rabbit pAb (20 μl)

NFIL3 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A14029
Availability: In Stock
$108.00
-+
Reactivity: Human, Rat    
Applications: WB, IF/IC, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Polyclonal antibody    
Gene Name: nuclear factor, interleukin 3 regulated
Gene Symbol: NFIL3
Synonyms: E4BP4; IL3BP1; NFIL3A; NF-IL3A; NFIL3
Gene ID: 4783
UniProt ID: Q16649
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 133-462 of human NFIL3 (NP_005375.2).
Dilution: WB 1:500-1:1000; IF/IC 1:50-1:100
Purification Method: Affinity purification
Concentration: 0.26 mg/ml
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze/thaw cycles.
Documents: Manual-NFIL3 antibody




Background

The protein encoded by this gene is a transcriptional regulator that binds as a homodimer to activating transcription factor (ATF) sites in many cellular and viral promoters. The encoded protein represses PER1 and PER2 expression and therefore plays a role in the regulation of circadian rhythm. Three transcript variants encoding the same protein have been found for this gene.




Images

FKRP antibodyWestern blot analysis of lysates from mouse liver, using NFIL3 Rabbit pAb (A14029) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.
Immunofluorescence analysis of MCF-7 cells using NFIL3 Rabbit pAb (A14029). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.



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