Reactivity: | Human, Rat |
Applications: | WB, IF/IC, ELISA |
Host Species: | Rabbit |
Isotype: | IgG |
Clonality: | Polyclonal antibody |
Gene Name: | nuclear factor, interleukin 3 regulated |
Gene Symbol: | NFIL3 |
Synonyms: | E4BP4; IL3BP1; NFIL3A; NF-IL3A; NFIL3 |
Gene ID: | 4783 |
UniProt ID: | Q16649 |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 133-462 of human NFIL3 (NP_005375.2). |
Dilution: | WB 1:500-1:1000; IF/IC 1:50-1:100 |
Purification Method: | Affinity purification |
Concentration: | 0.26 mg/ml |
Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Documents: | Manual-NFIL3 antibody |
Background
The protein encoded by this gene is a transcriptional regulator that binds as a homodimer to activating transcription factor (ATF) sites in many cellular and viral promoters. The encoded protein represses PER1 and PER2 expression and therefore plays a role in the regulation of circadian rhythm. Three transcript variants encoding the same protein have been found for this gene.
Images
![]() | Western blot analysis of lysates from mouse liver, using NFIL3 Rabbit pAb (A14029) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
![]() | Immunofluorescence analysis of MCF-7 cells using NFIL3 Rabbit pAb (A14029). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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