NTPCR Rabbit pAb (20 μl)

NTPCR Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A14944
Availability: In Stock
$108.00
-+
Reactivity:   Human, Mouse    
Applications:   WB, IF/IC, ELISA    
Host Species:   Rabbit    
Clonality:   Polyclonal antibody    
Gene Name:   nucleoside-triphosphatase, cancer-related
Gene Symbol:   NTPCR
Synonyms:   THEP1; C1orf57; HCR-NTPase; NTPCR
Gene ID:   84284
UniProt ID:   Q9BSD7
Immunogen:   Recombinant fusion protein containing a sequence corresponding to amino acids 91-190 of human NTPCR (NP_115700.1).
Dilution:   WB 1:500-1:2000; IF/IC 1:50-1:200
Purification Method:   Affinity purification
Concentration:   2.10 mg/ml
Buffer:   Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.
Documents: Manual-NTPCR antibody




Background

The protein encoded by this gene is a non-specific nucleoside triphosphatase that is slow-acting in vitro. This gene is overexpressed in many tumor tissues, and while it is not essential for the cell, overexpression is cytotoxic. However, the cytotoxicity is not related to its triphosphatase activity.




Images

NTPCR antibodyWestern blot analysis of various lysates using NTPCR Rabbit pAb (A14944) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunofluorescence analysis of L929 cells using NTPCR Rabbit pAb (A14944) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.



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