NUP54 Rabbit pAb (20 μl)

NUP54 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A15454
Availability: In Stock
$108.00
-+
Reactivity:   Human, Mouse, Rat    
Applications:   WB, ELISA    
Host Species:   Rabbit    
Clonality:   Polyclonal antibody    
Gene Name:   nucleoporin 54
Gene Symbol:   NUP54
Synonyms:   NUP54
Gene ID:   53371
UniProt ID:   Q7Z3B4
Immunogen:   Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human NUP54 (NP_059122.2).
Dilution:   WB 1:200-1:2000
Purification Method:   Affinity purification
Concentration:   1.42 mg/mL
Buffer:   Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.
Documents: Manual-NUP54 antibody




Background

The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. This gene encodes a member of the phe-gly (FG) repeat-containing nucleoporin subset. Multiple alternatively spliced transcript variants have been found for this gene.




Images

NUP54 antibodyWestern blot analysis of various lysates using NUP54 Rabbit pAb (A15454) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.



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