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| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Homeobox protein NANOG |
Synonyms: | NANOG, Nanog, Nanog homeobox, hNanog, Homeobox protein NANOG |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Nanog |
Uniprot ID: | Q80Z64 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. / Observed MW. | 42 kDa / 42 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 500-1: 1000 | （new born）brain, ovary |
IHC/IF | Mouse, Rat | 1: 500-1: 1500 | testis |
Background
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes.
Images
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Western blot analysis of nanog (GB11331) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin embedded rat testis using nanog (GB11331) at dilution of 1: 900 |
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Immunohistochemistry analysis of paraffin embedded mouse testis using nanog (GB11331) at dilution of 1: 900 |
| Immunofluorescent analysis of paraffin embedded rat testis using nanog (GB11331) at dilution of 1: 500 |
| Immunofluorescent analysis of paraffin embedded mouse testis using nanog (GB11331) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |