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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | poly(ADP-ribose) polymerase family member 8 |
| Gene Symbol: | PARP8 |
| Synonyms: | ARTD16; pART16; PARP8 |
| Gene ID: | 79668 |
| UniProt ID: | Q8N3A8 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PARP8 (NP_001171527.1). |
| Dilution: | WB 1:500-1:2000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.454 mg/mL |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-PARP8 antibody |
Background
Enables protein ADP-ribosylase activity. Involved in protein auto-ADP-ribosylation and protein mono-ADP-ribosylation.
Images
 | Western blot analysis of various lysates using PARP8 Rabbit pAb (A12894) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry analysis of paraffin-embedded Human placenta using PARP8 Rabbit pAb (A12894) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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