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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | phosphatidylinositol 3-kinase catalytic subunit type 3 |
| Gene Symbol: | PIK3C3 |
| Synonyms: | VPS34; Vps34; hVps34; PIK3C3/VPS34 |
| Gene ID: | 5289 |
| UniProt ID: | Q8NEB9 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 130-412 of human PIK3C3/VPS34 (NP_002638.2). |
| Dilution: | WB 1:100-1:500; IHC 1:100-1:200; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.6mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-PIK3C3 antibody |
Background
Enables 1-phosphatidylinositol-3-kinase activity. Involved in early endosome to late endosome transport and regulation of cytokinesis. Acts upstream of or within autophagy and protein lipidation. Located in autolysosome; late endosome; and midbody.
Images
 | Western blot analysis of various lysates using PIK3C3/VPS34 Rabbit pAb (A12483) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of NIH/3T3 cells using PIK3C3/VPS34 Rabbit pAb (A12483) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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