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Reactivity: | Rat |
Applications: | WB, ELISA |
Host Species: | Rabbit |
Clonality: | Polyclonal antibody |
Gene Name: | phospholipase C eta 1 |
Gene Symbol: | PLCH1 |
Synonyms: | HPE14; PLCL3; PLC-L3; PLC eta 1; PLCH1 |
Gene ID: | 23007 |
UniProt ID: | Q4KWH8 |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1100-1400 of human PLCH1 (NP_055811.1). |
Dilution: | WB 1:1000-1:3000 |
Purification Method: | Affinity purification |
Concentration: | 0.96 mg/ml |
Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Documents: | Manual-PLCH1 antibody |
Background
PLCH1 is a member of the PLC-eta family of the phosphoinositide-specific phospholipase C (PLC) superfamily of enzymes that cleave phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) to generate second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) (Hwang et al., 2005 [PubMed 15702972]).
Images
![]() | Western blot analysis of lysates from rat brain, using PLCH1 Rabbit pAb (A12903) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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