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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | RNA polymerase I subunit E |
| Gene Symbol: | POLR1E |
| Synonyms: | A49; PAF53; PRAF1; RPA49; POLR1E |
| Gene ID: | 64425 |
| UniProt ID: | Q9GZS1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-419 of human POLR1E (NP_071935.1). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.38 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-POLR1E antibody |
Background
Predicted to enable DNA binding activity; DNA-directed 5'-3' RNA polymerase activity; and RNA polymerase I general transcription initiation factor binding activity. Involved in nucleolar large rRNA transcription by RNA polymerase I. Located in fibrillar center and nucleoplasm.
Images
 | Western blot analysis of various lysates using POLR1E Rabbit pAb (A12700) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunoprecipitation analysis of 200 μg extracts of Jurkat cells, using 3 μg POLR1E antibody (A12700). Western blot was performed from the immunoprecipitate using POLR1E antibody (A12700) at a dilution of 1:1000. |
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