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| Reactivity: | Human, Mouse |
| Applications: | WB, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | RNA polymerase II, I and III subunit H |
| Gene Symbol: | POLR2H |
| Synonyms: | RPB8; RPB17; RPABC3; POLR2H |
| Gene ID: | 5437 |
| UniProt ID: | P52434 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human POLR2H (NP_006223.2). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 2.27 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-POLR2H antibody |
Background
The three eukaryotic RNA polymerases are complex multisubunit enzymes that play a central role in the transcription of nuclear genes. This gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of this gene.
Images
 | Western blot analysis of various lysates using POLR2H Rabbit pAb (A13339) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunoprecipitation analysis of 200 μg extracts of MCF7 cells using 1 μg POLR2H antibody (A13339). Western blot was performed from the immunoprecipitate using POLR2H antibody (A13339) at a dilution of 1:1000. |
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