Reactivity: | Human, Mouse, Rat |
Applications: | WB, IF/IC, ELISA |
Host Species: | Rabbit |
Clonality: | Polyclonal antibody |
Gene Name: | peroxiredoxin 5 |
Gene Symbol: | PRDX5 |
Synonyms: | PLP; ACR1; B166; PRXV; PMP20; PRDX6; prx-V; SBBI10; AOEB166; HEL-S-55; Peroxiredoxin 5 (PRDX5) |
Gene ID: | 25824 |
UniProt ID: | P30044 |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 53-214 of human Peroxiredoxin 5 (PRDX5) (NP_036226.2). |
Dilution: | WB 1:500-1:2000; IF/IC 1:20-1:50 |
Purification Method: | Affinity purification |
Concentration: | 2.42 mg/ml |
Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Documents: | Manual-PRDX5 antibody |
Background
This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein interacts with peroxisome receptor 1 and plays an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. The use of alternate transcription start sites is thought to result in transcript variants that use different in-frame translational start codons to generate isoforms that are targeted to the mitochondrion (isoform L) or peroxisome/cytoplasm (isoform S). Multiple related pseudogenes have been defined for this gene.
Images
![]() | Western blot analysis of various lysates using Peroxiredoxin 5 (PRDX5) Rabbit pAb (A1269) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
![]() | Immunofluorescence analysis of U2OS cells using Peroxiredoxin 5 (PRDX5) Rabbit pAb (A1269). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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