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| Reactivity: | Human, Mouse |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | DNA primase subunit 2 |
| Gene Symbol: | PRIM2 |
| Synonyms: | p58; PRIM2A; PRIM2 |
| Gene ID: | 5558 |
| UniProt ID: | P49643 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human PRIM2 (NP_000938.2). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.89 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-PRIM2 antibody |
Background
This gene encodes the 58 kilodalton subunit of DNA primase, an enzyme that plays a key role in the replication of DNA. The encoded protein forms a heterodimer with a 49 kilodalton subunit. This heterodimer functions as a DNA-directed RNA polymerase to synthesize small RNA primers that are used to create Okazaki fragments on the lagging strand of the DNA. Alternative splicing of this gene results in multiple transcript variants. This gene has a related pseudogene, which is also present on chromosome 6.
Images
 | Western blot analysis of various lysates using PRIM2 Rabbit pAb (A11633) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of lysates from mouse spleen, using PRIM2 Rabbit pAb (A11633) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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