Reactivity: | Human, Mouse, Rat |
Applications: | WB, IF/IC, ELISA |
Host Species: | Rabbit |
Isotype: | IgG |
Clonality: | Polyclonal antibody |
Gene Name: | proteasome 26S subunit, non-ATPase 14 |
Gene Symbol: | PSMD14 |
Synonyms: | PAD1; POH1; RPN11; PSMD14 |
Gene ID: | 10213 |
UniProt ID: | O00487 |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 160-300 of human PSMD14 (NP_005796.1). |
Dilution: | WB 1:500-1:2000; IF/IC 1:50-1:100 |
Purification Method: | Affinity purification |
Concentration: | 2.37 mg/ml |
Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
Documents: | Manual-PSMD14 antibody |
Background
This gene encodes a component of the 26S proteasome. The 26S proteasome is a large multiprotein complex that catalyzes the degradation of ubiquitinated intracellular proteins. The encoded protein is a component of the 19S regulatory cap complex of the 26S proteasome and mediates substrate deubiquitination. A pseudogene of this gene is also located on the long arm of chromosome 2.
Images
![]() | Western blot analysis of various lysates using PSMD14 Rabbit pAb (A10782) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
![]() | Immunofluorescence analysis of NIH-3T3 cells using PSMD14 Rabbit pAb (A10782) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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