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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | RAB7A, member RAS oncogene family |
| Gene Symbol: | RAB7A |
| Synonyms: | RAB7; CMT2B; PRO2706 |
| Gene ID: | 7879 |
| UniProt ID: | P51149 |
| Clone ID: | 5A10A4 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 108-207 of human RAB7 (NP_004628.4). |
| Dilution: | WB 1:55000-1:220000; IF/IC 1:100-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.5 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-RAB7A antibody |
Background
RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies.
Images
 | Western blot analysis of various lysates using RAB7 Rabbit mAb (A12308) at 1:55000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
 | Confocal imaging of NIH/3T3 cells using RAB7 Rabbit mAb (A12308, dilution 1:100) followed by a further incubation with ABflo® 488-conjugated Goat Anti-Rabbit IgG (H+L) (AS073, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with Cy3 Goat Anti-Mouse IgG (H+L) Ab (AS008, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x. |
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