RABIF Rabbit pAb (20 μl)

RABIF Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A15090
Availability: In Stock
$108.00
-+
Reactivity:   Human, Mouse, Rat    
Applications:   WB, IF/IC, ELISA    
Host Species:   Rabbit    
Isotype:   IgG    
Clonality:   Polyclonal antibody    
Gene Name:   RAB interacting factor
Gene Symbol:   RABIF
Synonyms:   MSS4; RASGFR3; RASGRF3; RABIF
Gene ID:   5877
UniProt ID:   P47224
Immunogen:   Recombinant fusion protein containing a sequence corresponding to amino acids 1-123 of human RABIF (NP_002862.2).
Dilution:   WB 1:500-1:2000; IF/IC 1:50-1:100
Purification Method:   Affinity purification
Concentration:   1.36 mg/ml
Buffer:   Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.
Documents: Manual-RABIF antibody




Background

This gene encodes a member of the SCE4/YPT1/RAB family of small GTP-binding proteins that are involved in the regulation of intracellular vesicular transport. This protein stimulates GTP-GDP exchange in SEC4, and to a lesser extent in YPT1 and RAB3A, and may play a general role in vesicular transport.




Images

RABIF antibodyWestern blot analysis of various lysates using RABIF Rabbit pAb (A15090) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
Immunofluorescence analysis of L929 cells using RABIF Rabbit pAb (A15090) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.



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