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| Reactivity: | Human, Mouse |
| Applications: | WB, IF/IC, IP, ELISA, ChIP |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | REST corepressor 1 |
| Gene Symbol: | RCOR1 |
| Synonyms: | RCOR; COREST; CoREST/RCOR1 |
| Gene ID: | 23186 |
| UniProt ID: | Q9UKL0 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 256-485 of human CoREST/CoREST/RCOR1 (NP_055971.2). |
| Dilution: | WB 1:500-1:2000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.44 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-RCOR1 antibody |
Background
This gene encodes a protein that is well-conserved, downregulated at birth, and with a specific role in determining neural cell differentiation. The encoded protein binds to the C-terminal domain of REST (repressor element-1 silencing transcription factor).
Images
 | Western blot analysis of various lysates using CoREST/RCOR1 Rabbit pAb (A12845) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg CoREST/RCOR1 antibody (A12845). Western blot was performed from the immunoprecipitate using CoREST/RCOR1 antibody (A12845) at a dilution of 1:1000. |
 | Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using CoREST/RCOR1 antibody (A12845) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
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