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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Mouse |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | RELA proto-oncogene, NF-kB subunit |
| Gene Symbol: | RELA |
| Synonyms: | p65; CMCU; NFKB3; AIF3BL3; NF-kB p65/RelA |
| Gene ID: | 5970 |
| UniProt ID: | Q04206 |
| Clone ID: | 8F9G2 |
| Immunogen: | Recombinant protein of human NF-kB p65 |
| Dilution: | WB 1:1000-1:2000; IF/IC 1:100-1:800 |
| Purification Method: | Affinity purification |
| Concentration: | 1 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-RELA antibody |
Background
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blot analysis of various lysates using NF-kB p65/RelA Mouse mAb (A10609) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of NIH/3T3 cells using NF-kB p65/RelA Mouse mAb (A10609) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of C6 cells using NF-kB p65/RelA Mouse mAb (A10609) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U2OS cells using NF-kB p65/RelA Mouse mAb (A10609) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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