RHD Rabbit pAb (20 μl)

RHD Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A15091
Availability: In Stock
$108.00
-+
Reactivity:   Human, Rat    
Applications:   WB, ELISA    
Host Species:   Rabbit    
Isotype:   IgG    
Clonality:   Polyclonal antibody    
Gene Name:   Rh blood group D antigen
Gene Symbol:   RHD
Synonyms:   RH; Rh4; RH30; RhII; RhPI; DIIIc; RHCED; RHDel; RHPII; RhDCw; CD240D; HDFNRH; RHXIII; RHDVA(TT); RhK562-II; RHD
Gene ID:   6007
UniProt ID:   Q02161
Immunogen:   A synthetic peptide corresponding to a sequence within amino acids 150-250 of human RHD (NP_001121163.1).
Dilution:   WB 1:500-1:2000
Purification Method:   Affinity purification
Concentration:   2.40 mg/ml
Buffer:   Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.
Documents: Manual-RHD antibody




Background

The Rh blood group system is the second most clinically significant of the blood groups, second only to ABO. It is also the most polymorphic of the blood groups, with variations due to deletions, gene conversions, and missense mutations. The Rh blood group includes this gene, which encodes the RhD protein, and a second gene that encodes both the RhC and RhE antigens on a single polypeptide. The two genes, and a third unrelated gene, are found in a cluster on chromosome 1. The classification of Rh-positive and Rh-negative individuals is determined by the presence or absence of the highly immunogenic RhD protein on the surface of erythrocytes. Multiple transcript variants encoding different isoforms have been found for this gene.




Images

RHD antibodyWestern blot analysis of various lysates using RHD Rabbit pAb (A15091) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.



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