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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | ribosomal protein L21 |
| Gene Symbol: | RPL21 |
| Synonyms: | L21; eL21; HYPT12; RPL21 |
| Gene ID: | 6144 |
| UniProt ID: | P46778 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human RPL21 (NP_000973.2). |
| Dilution: | WB 1:500-1:2000; IHC 1:50-1:100; IF/IC 1:50-1:100 |
| Purification Method: | Affinity purification |
| Concentration: | 0.65 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-RPL21 antibody |
Background
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L21E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Images
 | Western blot analysis of various lysates using RPL21 Rabbit pAb (A14254) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry analysis of paraffin-embedded Rat brain using RPL21 Rabbit pAb (A14254) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Confocal immunofluorescence analysis of HeLa cells using RPL21 Rabbit pAb (A14254) at dilution of 1:200. Blue: DAPI for nuclear staining. |
 | Confocal immunofluorescence analysis of A-431 cells using RPL21 Rabbit pAb (A14254) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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