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S6 Ribosomal Protein (RPS6) Rabbit mAb (20 μl)
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | ribosomal protein S6 |
| Gene Symbol: | RPS6 |
| Synonyms: | S6; eS6; S6 Ribosomal Protein (RPS6) |
| Gene ID: | 6194 |
| UniProt ID: | P62753 |
| Clone ID: | 4C7P4 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human RPS6 (NP_001001.2). |
| Dilution: | WB 1:1000-1:2000; IF/IC 1:100-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 1 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-RPS6 antibody |
Background
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Images
 | Western blot analysis of various lysates using S6 Ribosomal Protein (RPS6) Rabbit mAb (A11874) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Confocal imaging of NIH/3T3 cells using S6 Ribosomal Protein (RPS6) Rabbit mAb (A11874,dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x. |
 | Confocal imaging of C6 cells using S6 Ribosomal Protein (RPS6) Rabbit mAb (A11874,dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x. |
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