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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | ribonucleotide reductase catalytic subunit M1 |
| Gene Symbol: | RRM1 |
| Synonyms: | R1; RR1; RIR1; RRM1 |
| Gene ID: | 6240 |
| UniProt ID: | P23921 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 593-792 of human RRM1 (NP_001024.1). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:200; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.30 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-RRM1 antibody |
Background
This gene encodes the large and catalytic subunit of ribonucleotide reductase, an enzyme essential for the conversion of ribonucleotides into deoxyribonucleotides. A pool of available deoxyribonucleotides is important for DNA replication during S phase of the cell cycle as well as multiple DNA repair processes. Alternative splicing results in multiple transcript variants.
Images
 | Western blot analysis of various lysates using RRM1 Rabbit pAb (A1152) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of lysates from Rat lung, using RRM1 Rabbit pAb (A1152) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon using RRM1 Rabbit pAb (A1152) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunofluorescence analysis of HeLa cells using RRM1 Rabbit pAb (A1152) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200 μg extracts of HT-29 cells using 3 μg RRM1 antibody (A1152). Western blot was performed from the immunoprecipitate using RRM1 antibody (A1152) at a dilution of 1:1000. |
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