S100P Rabbit pAb (20 μl)

S100P Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A14780
Availability: In Stock
$108.00
-+
Reactivity: Human    
Applications: WB, IHC, IF/IC, IP,ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Polyclonal antibody    
Gene Name: S100 calcium binding protein P
Gene Symbol: S100P
Synonyms: MIG9; S100P
Gene ID: 6286
UniProt ID: P25815
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-95 of human S100P (NP_005971.1).
Dilution: WB 1:500-1:1000; IHC 1:100-1:200; IF/IC 1:50-1:200
Purification Method: Affinity purification
Concentration: 0.71 mg/mL
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze/thaw cycles.
Documents: Manual-S100P antibody




Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 4p16. This protein, in addition to binding Ca2+, also binds Zn2+ and Mg2+. This protein may play a role in the etiology of prostate cancer.




Images

MCM7 antibodyWestern blot analysis of various lysates using S100P Rabbit pAb (A14780) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Immunohistochemistry analysis of paraffin-embedded Human placenta using S100P Rabbit pAb (A14780) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of paraffin-embedded human placenta using S100P Rabbit pAb (A14780) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300 μg extracts of HT-29 cells using 3 μg S100P antibody (A14780). Western blot was performed from the immunoprecipitate using S100P antibody (A14780) at a dilution of 1:1000.



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