Reactivity: | Human |
Applications: | WB, IHC, IF/IC, IP,ELISA |
Host Species: | Rabbit |
Isotype: | IgG |
Clonality: | Polyclonal antibody |
Gene Name: | S100 calcium binding protein P |
Gene Symbol: | S100P |
Synonyms: | MIG9; S100P |
Gene ID: | 6286 |
UniProt ID: | P25815 |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-95 of human S100P (NP_005971.1). |
Dilution: | WB 1:500-1:1000; IHC 1:100-1:200; IF/IC 1:50-1:200 |
Purification Method: | Affinity purification |
Concentration: | 0.71 mg/mL |
Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Documents: | Manual-S100P antibody |
Background
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 4p16. This protein, in addition to binding Ca2+, also binds Zn2+ and Mg2+. This protein may play a role in the etiology of prostate cancer.
Images
![]() | Western blot analysis of various lysates using S100P Rabbit pAb (A14780) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
![]() | Immunohistochemistry analysis of paraffin-embedded Human placenta using S100P Rabbit pAb (A14780) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
![]() | Immunofluorescence analysis of paraffin-embedded human placenta using S100P Rabbit pAb (A14780) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
![]() | Immunoprecipitation analysis of 300 μg extracts of HT-29 cells using 3 μg S100P antibody (A14780). Western blot was performed from the immunoprecipitate using S100P antibody (A14780) at a dilution of 1:1000. |
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