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| Reactivity: | Human, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | sphingomyelin synthase 1 |
| Gene Symbol: | SGMS1 |
| Synonyms: | MOB; MOB1; SMS1; TMEM23; hmob33; SGMS1 |
| Gene ID: | 259230 |
| UniProt ID: | Q86VZ5 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-134 of human SGMS1 (NP_671512.1). |
| Dilution: | WB 1:500-1:2000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.90 mg/ml |
| Buffer: | Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-SGMS1 antibody |
Background
The protein encoded by this gene is predicted to be a five-pass transmembrane protein. This gene may be predominately expressed in brain.
Images
 | Western blot analysis of lysates from A-549 cells, using SGMS1 Rabbit pAb (A15008) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 930s. |
 | Immunofluorescence analysis of HeLa cells using SGMS1 Rabbit pAb (A15008) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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