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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | sirtuin 1 |
| Gene Symbol: | SIRT1 |
| Synonyms: | SIR2; SIR2L1; SIR2alpha; SIRT1 |
| Gene ID: | 23411 |
| UniProt ID: | Q96EB6 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 448-747 of human SIRT1 (NP_036370.2). |
| Dilution: | WB 1:1000-1:5000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 2.34 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-SIRT1 antibody |
Background
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.
Images
 | Western blot analysis of various lysates, using SIRT1 Rabbit pAb (A11267) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon using SIRT1 Rabbit pAb (A11267) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
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