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| Reactivity: | Human, Mouse |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | SRA stem-loop interacting RNA binding protein |
| Gene Symbol: | SLIRP |
| Synonyms: | DC50; PD04872; C14orf156; SLIRP |
| Gene ID: | 81892 |
| UniProt ID: | Q9GZT3 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SLIRP (NP_112487.1). |
| Dilution: | WB 1:200-1:2000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.16 mg/ml |
| Buffer: | Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-SLIRP antibody |
Background
Steroid receptor RNA activator (SRA, or SRA1; MIM 603819) is a complex RNA molecule containing multiple stable stem-loop structures that functions in coactivation of nuclear receptors. SLIRP interacts with stem-loop structure-7 of SRA (STR7) and modulates nuclear receptor transactivation (Hatchell et al., 2006 [PubMed 16762838]).
Images
 | Western blot analysis of various lysates using SLIRP Rabbit pAb (A15524) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using SLIRP Rabbit pAb (A15524) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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