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| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | sprouty RTK signaling antagonist 3 |
| Gene Symbol: | SPRY3 |
| Synonyms: | spry-3; SPRY3 |
| Gene ID: | 10251 |
| UniProt ID: | O43610 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human SPRY3 (NP_005831.1). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.95 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-SPRY3 antibody |
Background
Involved in negative regulation of MAPK cascade. Predicted to be located in membrane. Predicted to be active in cytosol.
Images
 | Western blot analysis of lysates from 293T cells, using SPRY3 Rabbit pAb (A14669) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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