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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | tryptophan 2,3-dioxygenase |
| Gene Symbol: | TDO2 |
| Synonyms: | TO; TDO; TPH2; TRPO; HYPTRP; TDO2 |
| Gene ID: | 6999 |
| UniProt ID: | P48775 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TDO2 (NP_005642.1). |
| Dilution: | WB 1:500-1:1000; IF/IC 1:100-1:500 |
| Purification Method: | Affinity purification |
| Concentration: | 6.8 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-TDO2 antibody |
Background
This gene encodes a heme enzyme that plays a critical role in tryptophan metabolism by catalyzing the first and rate-limiting step of the kynurenine pathway. Increased activity of the encoded protein and subsequent kynurenine production may also play a role in cancer through the suppression of antitumor immune responses, and single nucleotide polymorphisms in this gene may be associated with autism.
Images
 | Western blot analysis of various lysates using TDO2 Rabbit pAb (A13182) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Western blot analysis of lysates from HuH-7 cells using TDO2 Rabbit pAb (A13182) at 1:3000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of A-431 cells using TDO2 Rabbit pAb (A13182) at dilution of 1:500 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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