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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | transketolase |
| Gene Symbol: | TKT |
| Synonyms: | TK; TKT1; SDDHD; HEL107; HEL-S-48; TKT |
| Gene ID: | 7086 |
| UniProt ID: | P29401 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 250-350 of human TKT (NP_001055.1). |
| Dilution: | WB 1:1000-1:5000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 2.24 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-TKT antibody |
Background
This gene encodes a thiamine-dependent enzyme which plays a role in the channeling of excess sugar phosphates to glycolysis in the pentose phosphate pathway. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Images
 | Western blot analysis of various lysates, using TKT Rabbit pAb (A13553) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry analysis of paraffin-embedded Rat brain using TKT Rabbit pAb (A13553) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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