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| Reactivity: | Mouse |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | TLE family member 1, transcriptional corepressor |
| Gene Symbol: | TLE1 |
| Synonyms: | ESG; ESG1; GRG1; TLE-1; TLE1 |
| Gene ID: | 7088 |
| UniProt ID: | Q04724 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 256-355 of human TLE1 (NP_005068.2). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.5 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-TLE1 antibody |
Background
Enables identical protein binding activity and transcription corepressor activity. Involved in negative regulation of I-kappaB kinase/NF-kappaB signaling; negative regulation of anoikis; and regulation of gene expression. Located in cytosol and nucleoplasm. Part of beta-catenin-TCF complex.
Images
 | Western blot analysis of lysates from Mouse liver, using TLE1 Rabbit pAb (A13360) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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