Your shopping cart is empty!
| Reactivity: | Human, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | tyrosinase |
| Gene Symbol: | TYR |
| Synonyms: | ATN; CMM8; OCA1; OCA1A; OCAIA; SHEP3; Tyrosinase |
| Gene ID: | 7299 |
| UniProt ID: | P14679 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-340 of human Tyrosinase (NP_000363.1). |
| Dilution: | WB 1:500-1:2000; IHC 1:1000-1:5000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.17 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-TYR antibody |
Background
The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene.
Images
 | Western blot analysis of lysates from rat skin, using Tyrosinase Rabbit pAb (A13608) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry analysis of paraffin-embedded Human malignant melanoma tissue using Tyrosinase Rabbit pAb (A13608) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
You may also be interested in:
Related Products
-10%
-16%
