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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | U2 small nuclear RNA auxiliary factor 1 |
| Gene Symbol: | U2AF1 |
| Synonyms: | RN; FP793; U2AF35; U2AFBP; RNU2AF1; U2AF1 |
| Gene ID: | 7307 |
| UniProt ID: | Q01081 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human U2AF1 (NP_006749.1). |
| Dilution: | WB 1:500-1:2000; IHC 1:100-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.39 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-U2AF1 antibody |
Background
This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified.
Images
 | Western blot analysis of various lysates using U2AF1 Rabbit pAb (A13166) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
 | Immunohistochemistry analysis of paraffin-embedded Human lung cancer using U2AF1 Rabbit pAb (A13166) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg U2AF1 antibody (A13166). Western blot was performed from the immunoprecipitate using U2AF1 antibody (A13166) at a dilution of 1:1000. |
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